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DNA/Protein
Synthesis and Analysis Core Lab
protein
sequencer, peptide synthesizer, DNA synthesizer, HPLCs, Speed Vac system
IBB 3327, contact Nadia Boguslavsky 404-385-0215
Microscopy
and Microanalysis Instrument Facility
Multi-photon
excitation confocal microscope, flow cytometer/cell sorter, confocal
microscope, Coulter cell counter, fluorescence microplate reader, digital
flow cytometer, fluorescence/phospho-imager IBB 1328, contact Johnafel
Crowe 404-894-2212
DNA Sequencing
Core Lab
Cherry
Emerson, Georgia Tech, contact Brian Lynch 404-385-2844
Bioanalytical
Mass Spectrometry Core Facility
This facility
currently houses five mass spectrometers. Two of these instruments are
recently acquired state-of-the-art tandem mass spectrometers. These
instruments are an ABI QSTAR-XL of hybrid Q-TOF configuration and an
ABI 4700 MALDI TOF/TOF. These instruments provide a unique and complimentary
mass spectrometry capability with regard to both ionization and dissociation
of widely different molecular species.
The QSTAR
is equipped with capillary LC pumps, autosampler, as well as microswitching
and fractioning devices enabling analysis of complex mixtures with very
high sensitivity. The QSTAR has both an electrospray and dynamic nanospray
source for generation of intact molecular ions directly from solution.
Both MS and MS/MS data are acquired with high resolution (> 7000
FWHM) and accurate mass measurement (< 10 ppm) for unequivocal molecular
identification and structure elucidation. MS/MS on this instrument is
performed via low energy collisions (CID) analogous to that of a triple
quadrupole.
The TOF/TOF
generates ions via matrix assisted laser desorption (MALDI) with a 200Hz
laser. This enables either a 10-fold increase in sample throughput or
data collection vs other MALDI instruments. This instrument is equipped
with both linear and reflecting detectors. The former is used for large
molecular mass species (> 10 kDa) with low resolution, and the latter
is used for high resolution, accurate mass measurement of lower molecular
mass species (< 10 kDa). The ABI 4700 is a true tandem mass spectrometer
whereby MS/MS is performed via precursor ion selection with subsequent
dissociation by either metastable or high energy collision induced dissociation.
IBB, contact Dr. Cameron Sullards 404-894-4061.
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